Loop engineering in the thermophile cellobiohydrolase, Cel7A from Rasamsonia emersonii: Effects on activity and substrate interactions

Authors

  • Trine Holst S?rensen

Abstract

Cel7A cellobiohydrolases perform processive hydrolysis on one strand of cellulose, which is threaded through the enzyme?s substrate binding tunnel. The tunnel structure results from a groove in the catalytic domain, which is covered by a number of loops. These loops have been identified as potential targets for engineering of this industrially important enzyme family, but only few systematic studies on this have been made. In the current work we study two Asn residues in the B2 loop of the thermostable Cel7A from Rasamsonia emersonii. We found that Asn194 and particularly Asn197 promoted substrate binding, and the replacement of one or both of these. Forfattere: Trine Holst S?rensen, Michael S. Windahl, Jeppe Kari, Johan Olsen Kim Borch & Peter Westh (manuscript in preparation) DENNE ARTIKEL ER P? GRUND AF OPHAVSRETSM?SSIGE ?RSAGER DESV?RRE IKKE TILG?NGELIG Kontakt evt. forfatterne for at f? en kopi af artiklen. DUE TO THE LICENSE AGREEMENT WITH THE PUBLISHER, THIS ARTICLE IS NOT AVAILABLE Contact the authors for a copy of the article.

Published

2016-01-01

Issue

Test, nr. 2, 2016

Section

Articles

License

License to share

This Journal gives Open Access with CreativeCommons license CC-BY-NC-ND 4.0:

ccbyncnd

You can download all the content of the Journal and share it with others as long as you credit the authors and the journal, but you can’t change it in any way or use it commercially.

More specifically this license means that you – authors and users – may:

Share — copy and redistribute the material in any medium or form as long as you follow the license terms. The freedom to share includes parallel publishing on authors’ own website and in institutional repositories or in ResearchGate after publication.

You may share under these terms:

by

Attribution — You must give appropriate credit and provide a link to the license. Appropriate credit implies that you provide the name of the creator and attribution parties, a copyright notice, a license notice, a disclaimer notice, and a link to the material.

nc

NonCommercial — You may not use the material for commercial purposes. A commercial use is one primarily intended for commercial advantage or monetary compensation.

nd

No Derivatives — If you remix, transform, or build upon the material, you may not distribute the modified material. Merely changing the format never creates a derivative.

You can read specifically about the legal code behind this license here, and more generally about CreativeCommons licenses at their webpage.

Copyright of others
Authors are responsible for obtaining permission from copyright holders for reproducing any illustrations, tables, figures or lengthy quotations previously published elsewhere.